Get tips on using Mouse Reg1 Antibody to perform Immunohistochemistry Mouse - Reg1
Get tips on using Mouse Prolactin ELISA to perform ELISA Mouse - PRL
Get tips on using Mouse Adiponectin ELISA to perform ELISA Mouse - Adiponectin
Get tips on using QIAamp DNA Blood Mini Kit to perform DNA isolation / purification Cells - Immortalized cell lines HEK 293T
Get tips on using DNeasy Blood and Tissue Kit (250) to perform DNA isolation / purification Cells - Immortalized cell lines Loucy
Get tips on using Xfect™ Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human pulmonary artery smooth muscle cells (HPASMC)
Get tips on using Mouse ANGPTL3 ELISA to perform ELISA Mouse - Angiopoietin-Like 3 (AngptL3)
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
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