siRNA / RNAi /miRNA transfection Human Primary splenocytes

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using Double-negative T Cell Isolation Kit, human to perform Cell Isolation Double-negative T Cell Isolation

Get tips on using FITC anti-human/mouse Granzyme B Antibody to perform Flow cytometry Anti-bodies Mouse - Granzyme B

Get tips on using Lipofectamine® LTX Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines Neuro2a

Get tips on using Lipofectamine® LTX Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines HeLa

Get tips on using Lipofectamine® LTX Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines BXPC3

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rabbit skeletal muscle-derived stem cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary mouse pulmonary artery smooth muscle cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat pulmonary artery smooth muscle cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary porcine coronary artery smooth muscle cells