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TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Cellular assays TUNEL assay cell type PC-3 human prostate cancer

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Cellular assays TUNEL assay cell type HEK293 human embryonic kidney cells

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Cellular assays TUNEL assay cell type FaDu human squamous cell carcinoma

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - rabbit bone marrow mesenchymal stem cells

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Get tips on using ApoAlert™ DNA Fragmentation Assay Kit to perform TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma

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Get tips on using EpiQuik Dnmt3A Assay Kit to perform DNA methylation profiling Whole genome profiling - human peripheral blood mononuclear cells

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Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Rat - Megakaryocytes

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Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Rat - NRK52E

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Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Rat - Liver

Products Merck Millipore Chromatin Immunoprecipitation (ChIP) Assay Kit

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Rat - Pancreas

Products Merck Millipore Chromatin Immunoprecipitation (ChIP) Assay Kit

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