Site Directed Mutagenesis (SDM) Human Point mutation IMR-32

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Iris

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Human Seminal vesicles

Get tips on using ChIP Kit Magnetic - One Step (ab156907) to perform ChIP Human - MIA PaCa-2

Get tips on using ChIP Kit Magnetic - One Step (ab156907) to perform ChIP Human - MDA-MB-231

Get tips on using siRNA Wee1 to perform siRNA / miRNA gene silencing Human - SKOV-3 Wee-1

Get tips on using siRNA RBM3 to perform siRNA / miRNA gene silencing Human - MIA PaCa-2 RBM3

Get tips on using siRNA RIP3 to perform siRNA / miRNA gene silencing Human - MIA PaCa-2 RIP3

Get tips on using siRNA Wee1 to perform siRNA / miRNA gene silencing Human - MDA-MB-231 Wee1

Get tips on using siRNA FOXM1 to perform siRNA / miRNA gene silencing Human - MDA-MB-231 FOXM1

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.