rna-isolation-purification-cells-primary-rat-cardiac-fibroblasts

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Yeast - Schizosaccharomyces pombe

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Yeast - Coprinus cinereus

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Tissue - Mouse Tongue

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Tissue - Human Ovaries

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Tissue - Human Muscles

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Tissue - Human Joints

Get tips on using Biotin Rat Anti-Mouse Ly-6A/E to perform Flow cytometry Anti-bodies Mouse - Ly-6A-E/Sca1

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)