Protein expression and purification Insect cells S2

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary porcine airway epithelial cells

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - primary human bronchial epithelial cells

Get tips on using Quick-RNA Microprep Kit to perform RNA isolation / purification Cells - primary human aortic endothelial cells

Get tips on using NucleoBond® RNA/DNA to perform DNA isolation / purification Cells - Primary cells Rat cortical neurons

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Cells - Primary cells Rat cortical neurons

Get tips on using Quick-RNA™ MiniPrep Plus to perform RNA isolation / purification Cells - primary human epithelial cells

Get tips on using APC Rat Anti-Mouse Ly-6G and Ly-6C to perform Flow cytometry Anti-bodies Mouse - Ly6C/Gr-1/Ly6G

Get tips on using Dead Cell Apoptosis Kit with Annexin V FITC and PI to perform Apoptosis assay cell type - OECM-1

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Corning™ Basal Cell Culture Liquid Media - DMEM and Ham's F-12, 50/50 Mix to perform Mammalian cell culture media HSG cells