RNA isolation / purification Cells Cancer cell lines Leukemia cancer cell lines

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Mouse Mammary glands

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Mouse Lymph node

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Thyroid gland

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Spinal cord

Get tips on using Maxwell® 16 LEV simplyRNA Purification Kit to perform RNA isolation / purification Tissue - Human Muscles

Get tips on using Absolutely RNA FFPE Kit to perform RNA isolation / purification Tissue - Human Kidney

Get tips on using NucleoSpin® RNA Plus to perform RNA isolation / purification Tissue - Mouse Retina

Get tips on using Total RNA Kit, peqGOLD to perform RNA isolation / purification Tissue - Human Stomach