RNA isolation / purification Cells immortalized

Get tips on using Gentra Puregene Yeast/Bact. Kit to perform DNA isolation / purification Yeast - Candida albicans

Get tips on using QIAamp DNA Blood Midi Kit to perform DNA isolation / purification Tissue - blood / plasma

Get tips on using QIAamp DNA Stool Mini Kit to perform DNA isolation / purification Yeast - Saccharomyces boulardii

Get tips on using MagAttract Direct mRNA M48 Kit (192) to perform mRNA / Ribonucleoprotein isolation / purification mRNA

Get tips on using DNeasy PowerClean Pro Cleanup Kit (50) to perform DNA isolation / purification Water samples

Get tips on using cobas® DNA Sample Preparation Kit to perform DNA isolation / purification Tissue - lung

Get tips on using High Pure PCR Template Preparation Kit to perform DNA isolation / purification Tissue - placenta

Get tips on using Dynabeads™ Untouched™ Human B Cells Kit to perform Cell Isolation B cell

I am currently using a recombinant protein which shows metal-dependent DNase activity. Is it possible to pinpoint the source of the DNase activity after protein purification? More specifically, can I ensure that the DNase activity is not because of nuclease contamination from the E.coli that might have persisted and passed with the protein of interest during purification?

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.