An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using CM-H2DCFDA (General Oxidative Stress Indicator) to perform ROS assay cell type - PLHC-1, SK-HEP-1, Hep3b, HepG2 human hepatocellular carcinoma
Get tips on using Carboxy-H2DCFDA (general oxidative stress indicator) to perform ROS assay cell type - PLHC-1, SK-HEP-1, Hep3b, HepG2 human hepatocellular carcinoma
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Human Kidney
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Human Liver
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Human Veins
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Human Tonsil
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Human Prostate
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Human Muscles
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