DNA methylation profiling Gene specific profiling MRC-5

Get tips on using Dusp3 siRNA to perform siRNA / miRNA gene silencing Mouse - BMDMs Dusp3

Get tips on using Dusp3 siRNA to perform siRNA / miRNA gene silencing Mouse - RAW264.7 Dusp3

Get tips on using AllStars Hs Cell Death siRNA to perform siRNA / miRNA gene silencing Human - U2OS KRAS

Get tips on using PACE4 siRNA (h) to perform siRNA / miRNA gene silencing Human - DuCaP

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification MCF10A breast epithelial cells

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using QIAamp Media MDx Kit (12) to perform DNA isolation / purification Tissue - genital / cervical samples

Get tips on using EpiTect Bisulfite Kit to perform Bisulfite DNA Modification Fluids

Get tips on using FlexiTube GeneSolution GS27279 for Tnfrsf12a to perform siRNA / miRNA gene silencing Mouse - B16-BL6 FN14/Tnfrsf12a

Get tips on using FlexiTube GeneSolution GS26354 for GNL3 to perform siRNA / miRNA gene silencing Human - MDA-MB-231 GNL3