rna-isolation-purification-tissue-mouse-colon

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Pierce™ Coomassie (Bradford) Protein Assay Kit to perform Protein quantification Tissue - mouse liver

Get tips on using Pierce™ Coomassie (Bradford) Protein Assay Kit to perform Protein quantification Tissue - mouse kidney

Get tips on using Pierce™ Coomassie (Bradford) Protein Assay Kit to perform Protein quantification Tissue - mouse brain

Get tips on using Mouse VEGF ELISA Kit (ab100751) to perform ELISA Mouse - VEGF

Get tips on using Mouse VEGF Quantikine ELISA Kit to perform ELISA Mouse - VEGF

Get tips on using Mouse Shh-N ELISA Kit to perform ELISA Mouse - ShhN

Get tips on using RBP4 ELISA Kit (Mouse) (OKBB00339) to perform ELISA Mouse - RBP4

Get tips on using Mouse RBP4 Quantikine ELISA Kit to perform ELISA Mouse - RBP4

Get tips on using Mouse Prolactin ELISA Kit (ab100736) to perform ELISA Mouse - PRL