rna-isolation-purification-tissue-rat-bronchi

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using FlashTag™ Biotin HSR RNA Labeling Kits to perform Microarray RNA amplification & Labeling - Mouse skin tissue Biotin

Get tips on using FlashTag™ Biotin HSR RNA Labeling Kits to perform Microarray RNA amplification & Labeling - Mouse mammary tissue Biotin

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - primary porcine coronary artery endothelial cells

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - primary porcine coronary artery endothelial cells

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Cells - primary human umbilical vein endothelial cells

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Cells - primary human dermal microvascular endothelial cells

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary mouse dorsal root ganglion neurons

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary human aortic smooth muscle cells