siRNA / miRNA gene silencing Human HL-60

Get tips on using EpiTect Fast DNA Bisulfite Kit (50) to perform DNA methylation profiling Gene specific profiling - Hep3B SPRY1

Get tips on using Luminescent β-galactosidase Detection Kit II to perform Reporter gene assay β-galactosidase substrates - RAW 264.7

Get tips on using Negative control, native pGL4.13 luciferase vector to perform Reporter gene assay luciferase - negative control (luciferase vector)

Get tips on using Secrete-Pair™ Dual Luminescence Assay Kit to perform Reporter gene assay β-galactosidase substrates - HUVEC

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using pLX-sgRNA to perform CRISPR Human - Deletion CIITA

Get tips on using MethylEasy™ Xceed (ME002) to perform DNA methylation profiling Gene specific profiling - Rat whole pituitary glands PROP1

Get tips on using EZ DNA Methylation-Direct Kit to perform DNA methylation profiling Gene specific profiling - A2780 miR-30a-5p

Get tips on using EZ DNA Methylation-Direct Kit to perform DNA methylation profiling Gene specific profiling - A2780 miR-30c-5p

Get tips on using CpGenome Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - Ca Ski HPV-16