Get tips on using Annexin V-FITC Kit to perform Apoptosis assay cell type - OVCAR-3
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using pGB2B3 to perform Protein Expression Prokaryotic cells - E. coli hβ-defensin 2/3
Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized Caov-3
Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized bEnd.3
Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized PC-3
Get tips on using Direct-zol RNA Kits to perform RNA isolation / purification Cells - immortalized PC-3
Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized Calu-3
Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay SK-OV-3
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