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Protein isolation Mammalian cells

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ROS assay cell type - K562 human leukemia cells Experiment

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Cellular assays ROS assay cell type K562 human leukemia cells

Some help with RNA isolation using Trizol Discussion

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Discussions Some help with RNA isolation using Trizol

TRIzol Reagent Product

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized SHG44