rna-isolation-purification-tissue-mouse-spine

Get tips on using Mouse Lipocalin-2 ELISA Kit (NGAL) (ab199083) to perform ELISA Mouse - NGAL/LCN2

Get tips on using Mouse KIM1 ELISA Kit (TIM-1) (ab119596) to perform ELISA Mouse - KIM-1

Get tips on using Mouse IGF-1 PicoKine™ ELISA Kit to perform ELISA Mouse - IGF-I

Get tips on using Mouse Heme Oxygenase 1 ELISA Kit (ab204524) to perform ELISA Mouse - HO-1

Get tips on using Mouse BMP-2 PicoKine™ ELISA Kit to perform ELISA Mouse - BMP-2

Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray Gene expression arrays - Mouse brain tissue Biotin

Get tips on using Mouse TGF beta 1 ELISA Kit (ab119557) to perform ELISA Mouse - TGF-beta 1

Get tips on using Mouse IL-1 beta ELISA Kit (ab100704) to perform ELISA Mouse - IL-1 beta

Get tips on using Mouse ICAM-1/CD54 Quantikine ELISA Kit to perform ELISA Mouse - ICAM-1/CD54

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.