RNA isolation / purification Tissue Mouse

Get tips on using MethylFlash™ Methylated DNA Quantification Kit to perform DNA methylation profiling Whole genome profiling - mouse hippocampal tissue

Get tips on using β-Galactosidase Reporter Gene Staining Kit to perform Reporter gene assay β-galactosidase substrates - mouse embryo tissue

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Mouse/Rat Leptin Quantikine ELISA Kit to perform ELISA Rat - Leptin

Get tips on using Purified anti-mouse TCR γ/δ Antibody to perform Flow cytometry Anti-bodies Mouse - TCRγ/δ

Get tips on using PE/Cyanine7 anti-mouse CD252 (OX40L) Antibody to perform Flow cytometry Anti-bodies Mouse - CD252/OX40L

Get tips on using FITC anti-human/mouse Granzyme B Antibody to perform Flow cytometry Anti-bodies Mouse - Granzyme B

Get tips on using PE/Cyanine7 anti-mouse F4/80 Antibody to perform Flow cytometry Anti-bodies Mouse - F4/80

Get tips on using FITC anti-mouse CD326 (Ep-CAM) Antibody to perform Flow cytometry Anti-bodies Mouse - CD326/EpCAM