siRNA / RNAi /miRNA transfection Human Cells THP-1

Get tips on using APC-H7 Mouse Anti-Human CD44 to perform Flow cytometry Anti-bodies Human - CD44

Get tips on using CD133/2 Antibody, anti-human, APC to perform Flow cytometry Anti-bodies Human - CD133

Get tips on using CD133/2 Antibody, anti-human, PE to perform Flow cytometry Anti-bodies Human - CD133

Get tips on using Human Angiopoietin-like 3 DuoSet ELISA to perform ELISA Human - Angiopoietin-Like 3 (AngptL3)

Get tips on using Human Genome CGH Microarray Kit, 4x44K to perform Microarray Comperative genomic hybridization - Human HeLa

Get tips on using Human Genome CGH Microarray 244A Supplemental to perform Microarray Comperative genomic hybridization - Human STUMP

Get tips on using Human Genome CGH Microarray Kit 244A to perform Microarray Comperative genomic hybridization - Human HepG2

Get tips on using RIPA Buffer (10X) to perform Protein isolation Mammalian cells - Human gingival epithelial cells

Get tips on using Human EGFR In-Cell ELISA Kit (ab126419) to perform ELISA Human - EGFR

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.