Get tips on using Rat GE 4x44K v3 Microarray Kit to perform Microarray Gene expression arrays - Rat cholangio carcinoma cyanine 3 & cyanine 5
Get tips on using β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer to perform Reporter gene assay β-galactosidase substrates - CHO-K1
Get tips on using β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer to perform Reporter gene assay β-galactosidase substrates - COS-7
Get tips on using SurePrint G3 Mouse Exon 4x180K Microarray Kit (165,984 Exon probes) to perform Microarray Gene expression arrays - Mouse Cyanine-CTP
Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - PANC-1
Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - MCF-7
Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - SH-SY5Y
Get tips on using MitoSOX™ Red Mitochondrial Superoxide Indicator, for live-cell imaging to perform ROS assay cell type - A549 human adenocarcinomic human alveolar basal epithelial cells
Get tips on using β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer to perform Reporter gene assay β-galactosidase substrates - SK-Hep-1
DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
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