rna-isolation-purification-cells-primary-mouse-ventricles

Get tips on using Mouse IL-1 beta ELISA Kit (ab100704) to perform ELISA Mouse - IL-1 beta

Get tips on using Mouse ICAM-1/CD54 Quantikine ELISA Kit to perform ELISA Mouse - ICAM-1/CD54

Get tips on using Mouse C Reactive Protein ELISA Kit (PTX1) (ab157712) to perform ELISA Mouse - C-Reactive Protein/CRP

Plasmid isolation is an important technique in molecular biology or any kind of genetic editing. It involves amplifying plasmids overnight by transforming them into competent bacterial cells. The desired colonies of these bacteria can then be grown in shaker cultures, at appropriate shaking speed, oxygen availability and temperature. These liquid cultures can then be ultracentrifuged to pellet the bacteria, which are then used for plasmid isolation. The bacteria are first resuspended in a buffer, then lysed, neutralized, purified in a column, eluted, precipitated with ethanol and then resuspended. During plasmid isolation, it is important to lyse cells quickly because lysing bacteria for too long may lead to irreversible denaturing of the plasmid. Usually, alkaline lysis is used for isolation because it is a mild treatment. It isolates plasmid DNA and other cell components such as proteins by breaking cells apart with an alkaline solution. Precipitation removes the proteins, and the plasmid DNA recovers with alcohol precipitation. Resuspension and lysis buffers should be mixed thoroughly in order to prevent the DNA from breaking into smaller fragments. This is because broken gDNA can reanneal and remain in the solution, without binding to the column.

Get tips on using Purified Anti-Mouse TCR beta (H57-597) to perform Flow cytometry Anti-bodies Mouse - TCRbeta

Get tips on using Purified Hamster Anti-Mouse TCR β Chain to perform Flow cytometry Anti-bodies Mouse - TCRbeta

Get tips on using Purified anti-mouse CD115 (CSF-1R) Antibody to perform Flow cytometry Anti-bodies Mouse - CD115

Get tips on using Purified NA/LE Hamster Anti-Mouse CD40 to perform Flow cytometry Anti-bodies Mouse - CD40

Get tips on using Mouse Serpin E1/PAI-1 DuoSet ELISA to perform ELISA Mouse - Serpin E1/PAI-1

Get tips on using SurePrint G3 Mouse GE 8x60K Microarray Kit to perform Microarray Comperative genomic hybridization - Mouse iPSC