rna-isolation-purification-cells-primary-canine-peripheral-blood-mononuclear-cells

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Tissue - Mouse Artery / aorta

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Tissue - Mouse Mammary glands

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Tissue - Human Fallopian tubes

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Tissue - mouse aorta tissue

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Tissue - human placental tissue

Get tips on using TRIzol™ LS Reagent to perform RNA isolation / purification Tissue - human brain tissue

Get tips on using MEBMTM Mammary Epithelial Cell Growth Basal Medium to perform 3D Cell Culture Media Mouse primary breast ephitelial cells-Mammospheres

Get tips on using MEBMTM Mammary Epithelial Cell Growth Basal Medium to perform 3D Cell Culture Media Human primary breast ephitelial cells-Mammospheres

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform Stem cell culture media NCH421K cells primary glioma