Site Directed Mutagenesis (SDM) Human Point mutation SH-SY5Y

Get tips on using Apo-ONE® Homogeneous Caspase-3/7 Assay to perform Apoptosis assay cell type - SH-SY5Y

Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Immortalized cell lines SH-SY5Y

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - SH-SY5Y

Get tips on using TaKaRa MiniBEST Universal Genomic DNA Extraction Kit to perform DNA isolation / purification Cells - Immortalized cell lines SH-SY5Y

Get tips on using FitAmp Blood and Cultured Cell DNA Extraction Kit to perform DNA isolation / purification Cells - Immortalized cell lines SH-SY5Y

Get tips on using QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric to perform Cell migration / Invasion cell type - SH-SY5Y

Get tips on using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) to perform Cell cytotoxicity / Proliferation assay cell type - SH-SY5Y

Get tips on using Purified Mouse Anti-p62 Ick ligand Clone 3/P62 LCK LIGAND (RUO) to perform Autophagy assay cell type - SH-SY5Y

Get tips on using Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 & Propidium Iodide (PI) to perform Apoptosis assay cell type - SH-SY5Y