An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using Mouse/Rat Leptin Quantikine ELISA Kit to perform ELISA Rat - Leptin
Get tips on using Purified anti-mouse TCR γ/δ Antibody to perform Flow cytometry Anti-bodies Mouse - TCRγ/δ
Get tips on using PE/Cyanine7 anti-mouse CD252 (OX40L) Antibody to perform Flow cytometry Anti-bodies Mouse - CD252/OX40L
Get tips on using FITC anti-human/mouse Granzyme B Antibody to perform Flow cytometry Anti-bodies Mouse - Granzyme B
Get tips on using PE/Cyanine7 anti-mouse F4/80 Antibody to perform Flow cytometry Anti-bodies Mouse - F4/80
Get tips on using FITC anti-mouse CD326 (Ep-CAM) Antibody to perform Flow cytometry Anti-bodies Mouse - CD326/EpCAM
Get tips on using ON-TARGETplus Mouse Stat5b (20851) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - FL83B Stat5b
Get tips on using ON-TARGETplus Mouse Cdh5 (12562) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - MS1 Cdh5
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment