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Acid phosphatase detection heavily relies on determining the concentration of tartrate-resistant acid phosphatase (TRAP) in the sample. Hence, sample preparation is very crucial and it should be done strictly as per kit manufacturer instructions to avoid any inconsistency and poor sensitivity

Acid phosphatase detection heavily relies on determining the concentration of tartrate-resistant acid phosphatase (TRAP) in the sample. Hence, sample preparation is very crucial and it should be done strictly as per kit manufacturer instructions to avoid any inconsistency and poor sensitivity.

Get tips on using anti-alpha-Smooth Muscle Actin mouse monoclonal, ASM-1 to perform Immunohistochemistry Alpha smooth muscle Actin - Mouse -NA- -NA-

Get tips on using Anti-LC3B antibody (ab48394) to perform Autophagy assay cell type - A2058

Get tips on using Anti-LC3B antibody (ab48394) to perform Autophagy assay cell type - A375

Get tips on using Anti-TNF alpha antibody (ab6671) to perform Western blotting TNF-alpha

Get tips on using Anti-beta Actin antibody (ab8227) to perform Western blotting β-Actin

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Anti-AKT (phospho T308) antibody (ab38449) to perform Western blotting AKT

Get tips on using Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker (ab14748) to perform Western blotting ATP5A