rna-isolation-purification-tissue-mouse-spinal-cord

Get tips on using Mouse Von Willebrand Factor A2 ELISA Kit (ab208980) to perform ELISA Mouse - vWF-A2

Get tips on using Mouse heme oxygenase 1,HO-1 ELISA Kit to perform ELISA Mouse - HO-1

Get tips on using Q-Plex™ Mouse Cytokine – Inflammation (14-plex) to perform ELISA Mouse - GM-CSF

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - 3T3 L1 mouse adipose tissue

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Rat - Gingival tissue

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Rat - Liver tissue

Get tips on using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® to perform RNA sequencing Mouse - ESCs (Embryonic Stem Cells)

Get tips on using "Illumina ™ TotalPrep ™ RNA Amplification Kit + Bio-16-UTP (10 mM) to perform Microarray RNA amplification & Labeling - Mouse cochlaea Biotin

Plasmid isolation is an important technique in molecular biology or any kind of genetic editing. It involves amplifying plasmids overnight by transforming them into competent bacterial cells. The desired colonies of these bacteria can then be grown in shaker cultures, at appropriate shaking speed, oxygen availability and temperature. These liquid cultures can then be ultracentrifuged to pellet the bacteria, which are then used for plasmid isolation. The bacteria are first resuspended in a buffer, then lysed, neutralized, purified in a column, eluted, precipitated with ethanol and then resuspended. During plasmid isolation, it is important to lyse cells quickly because lysing bacteria for too long may lead to irreversible denaturing of the plasmid. Usually, alkaline lysis is used for isolation because it is a mild treatment. It isolates plasmid DNA and other cell components such as proteins by breaking cells apart with an alkaline solution. Precipitation removes the proteins, and the plasmid DNA recovers with alcohol precipitation. Resuspension and lysis buffers should be mixed thoroughly in order to prevent the DNA from breaking into smaller fragments. This is because broken gDNA can reanneal and remain in the solution, without binding to the column.

Get tips on using Mouse TGF Beta 1 PicoKine™ ELISA Kit to perform ELISA Mouse - TGF-beta 1