RNA isolation / purification Cells Cancer cell lines Leukemia cancer cell lines

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Get tips on using Live/Dead cell Staining Kit II to perform Live / Dead assay mammalian cells - SH-SY5Y Human neuroblastoma

Products PromoKine Live/Dead cell Staining Kit II

Get tips on using LIVE/DEAD™ Cell Imaging Kit to perform Live / Dead assay mammalian cells - SH-SY5Y Human neuroblastoma

Products Thermo Fisher Scientific LIVE/DEAD™ Cell Imaging Kit

Get tips on using Live/Dead cell Staining Kit II to perform Live / Dead assay mammalian cells - bmMSCs human bone marrow

Products PromoKine Live/Dead cell Staining Kit II

Get tips on using EasySep™ Human Monocyte Isolation Kit to perform Cell Isolation Monocyte

Products STEMCELL technologies EasySep™ Human Monocyte Isolation Kit

Get tips on using RealTime-Glo™ MT Cell Viability Assay to perform Live / Dead assay mammalian cells - HEK 293

Products Promega RealTime-Glo™ MT Cell Viability Assay

Get tips on using RealTime-Glo™ MT Cell Viability Assay to perform Live / Dead assay mammalian cells - THP-1

Products Promega RealTime-Glo™ MT Cell Viability Assay

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Cellular assays TUNEL assay cell type Rabbit synovial fibroblasts

Get tips on using EBMTM-2 Endothelial Cell Growth Basal Medium-2 to perform Stem cell culture media Cord blood-derived endothelial cells(hCBiPS2)

Products Lonza EBMTM-2 Endothelial Cell Growth Basal Medium-2

Get tips on using Mesenchymal Stem Cell Growth Medium to perform Stem cell culture media Human bone mesenchymal stem cell (BMSC)

Products Cyagen US Inc. Mesenchymal Stem Cell Growth Medium

Get tips on using Rat Endothelial Cell Growth Medium to perform Mammalian cell culture media RAOEC

Products Cell Applications Inc Rat Endothelial Cell Growth Medium

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