siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - U2OS (human bone osteosarcoma epithelial cells)

Get tips on using HyClone Minimal Essential Medium (MEM) variations: Liquid to perform Stem cell culture media Human salivary gland stem cells

Get tips on using ApopTag Fluorescein in Situ Apoptosis Detection Kit to perform TUNEL assay cell type - HEK293 human embryonic kidney cells

Get tips on using TdT In Situ Apoptosis Detection Kit - Fluorescein to perform TUNEL assay cell type - HeLa cells human cervical cancer

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - K562 human leukemia cells

Get tips on using OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) to perform ROS assay cell type - K562 human leukemia cells

Get tips on using PE Annexin V Apoptosis Detection Kit with 7-AAD to perform Apoptosis assay cell type - Human T-cells

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform Stem cell culture media NCH421K cells primary glioma

Get tips on using BLOCK-iT™ Adenoviral RNAi Expression System, pAd/BLOCK-iT™-DEST RNAi Gateway Vector to perform shRNA gene silencing Mouse - P19 Foxm1