DNA transfection Mammalian cells Immortalized cell lines

Get tips on using Easy-Spin (DNA free) Total RNA Extraction Kit to perform RNA isolation / purification Bacteria - Gram negative Vibro parahaemolyticus

Get tips on using illustra GFX PCR DNA and Gel Band Purification Kits to perform

Get tips on using Gentra Puregene Blood Kit to perform DNA isolation / purification Cells - Primary cells CD4+ T cells

Get tips on using HyClone™ Dulbecco's Modified Eagles Medium to perform Mammalian cell culture media MDA-MB-231

Get tips on using Gibco™Ham's F-12 Nutrient Mix to perform Mammalian cell culture media PC-3

Get tips on using FGMTM-3 Cardiac Fibroblast Growth Medium-3 BulletKit to perform Mammalian cell culture media HCF

Get tips on using RPMI 1640, with L-Glutamine and 25mM HEPES to perform Mammalian cell culture media DU145

Get tips on using RPMI 1640, with L-Glutamine and 25mM HEPES to perform Mammalian cell culture media VCaP

Get tips on using McCoy's 5A Medium, ATCC® 30-2007™ to perform Mammalian cell culture media A253

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.