siRNA / miRNA gene silencing Human KLM-1

Get tips on using AmpFLSTR™ Identifiler™ PCR Amplification Kit to perform Cell line authentication Colon cancer cell line DLD-1

Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines PANC-1

Get tips on using PE-Cy™7 Rat Anti-Mouse CD31 to perform Flow cytometry Anti-bodies Mouse - CD31/Pecam-1

Get tips on using In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based to perform Cell cytotoxicity / Proliferation assay cell type - THP-1

Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - THP-1

Get tips on using Dead Cell Apoptosis Kit with Annexin V FITC and PI to perform Apoptosis assay cell type - OECM-1

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

Get tips on using PowerPlex® 18D System to perform Cell line authentication Human iPSC cells derived from human dermal fibroblasts

Get tips on using MethylEasy™ Xceed (ME002) to perform DNA methylation profiling Gene specific profiling - UMR-106 BMP2

Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - CHO-K1