Get tips on using Anti-Collagen X antibody (ab58632) to perform Immunohistochemistry Mouse - Col X
Get tips on using Anti-Collagen III antibody (ab7778) to perform Western blotting Type III collagen
Get tips on using Immun-Star Goat Anti-Mouse (GAM)-HRP Conjugate to perform Western blot Secondary Antibody - Goat Mouse Horseradish peroxidase
Get tips on using Goat Anti-Rabbit IgG (H + L)-HRP Conjugate to perform Western blot Secondary Antibody - Goat Rabbit Horseradish peroxidase
Get tips on using BioMag Goat Anti-Rat IgG (500 ml) to perform Cell Isolation Mouse T cells
Get tips on using Cultrex® Collagen IV Cell Invasion Assay to perform Cell migration / Invasion cell type - TPC1
Get tips on using Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 568 conjugate to perform Flowcytometry Secondary Antibody - Goat Mouse Alexa Fluor 568
Get tips on using Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate to perform Flowcytometry Secondary Antibody - Goat Rabbit Alexa Fluor 488
Get tips on using COL1A Antibody (COL-1): sc-59772 to perform Western blotting Type I collagen
A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
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