Immunohistochemistry Collagen VII [II-32]

Get tips on using Cultrex® Collagen IV Cell Invasion Assay to perform Cell migration / Invasion cell type - TPC1

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using 96-Well Cell Invasion Assay, Collagen I to perform Cell migration / Invasion cell type - MCF-10A

Get tips on using 96-Well Cell Invasion Assay, Collagen I to perform Cell migration / Invasion cell type - MDA-MB-231

Get tips on using II-II6B3 to perform Immunohistochemistry Mouse - Col II

Get tips on using Oris™ Cell Migration Assay - Collagen I Coated to perform Cell migration / Invasion cell type - HaCat

Get tips on using Superscript reverse tran-scriptase II (SS RT II) system to perform cDNA synthesis Yeast

Get tips on using pFlpBtM-II-eGFP to perform Protein Expression Eukaryotic cells - S. frugiperda EGFP

Get tips on using CometAssay Electrophoresis System II to perform DNA Damage Assay HT1080

Get tips on using CometAssay Electrophoresis System II to perform DNA Damage Assay HeLa