Site Directed Mutagenesis (SDM) Human Deletion MDA-MB-231

Get tips on using Oris™ Pro Cell Migration Assay to perform Wound healing assay cell type - human MDA-MB-231

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using SNAI 1 siRNA and shRNA Plasmids (h) to perform siRNA / miRNA gene silencing Human - MDA-MB-231 SNAI 1

Get tips on using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® to perform RNA sequencing Human - MDA-MB-231

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized MDA-MB-231

Get tips on using GFP-CERTIFIED® Apoptosis/Necrosis detection kit to perform Necrosis MDA-MB-231

Get tips on using DMEM/F-12, no phenol red to perform 3D Cell Culture Media Human breast cancer MDA-MB-231 cells-Mammospheres

Get tips on using DMEM/F-12 PLUS Basal Medium to perform 3D Cell Culture Media Human breast cancer MDA-MB-231 cells-Mammospheres

Get tips on using Live-Dead Cell Staining Kit (BioVision) to perform Live / Dead assay mammalian cells - MDA-MB-231 human breast cancer cells

Get tips on using LIVE/DEAD™ Cell Imaging Kit to perform Live / Dead assay mammalian cells - MDA-MB-231 human breast cancer cells