PCR

Get tips on using Quick-DNA™ Fungal/Bacterial Miniprep Kit to perform DNA isolation / purification Yeast - Candida albicans

Get tips on using FastDNA™ SPIN Kit for Feces to perform DNA isolation / purification Bacteria - Gram positive Clostridium difficile

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized HeLa, CaSki and C33A (Cervical cancer cells)

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Cells - Immortalized cell lines Renal cortical tubule epithelial cells

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Bacteria - Gram positive piezophilic bacteria [AT7 and AT12 Strains]

Get tips on using Direct-zol™ RNA MiniPrep Plus to perform RNA isolation / purification Bacteria - Gram negative Escherichia coli

Get tips on using MICROBExpress™ Bacterial mRNA Enrichment Kit to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus aureus

Get tips on using RiboMinus™ Transcriptome Isolation Kit, bacteria to perform RNA isolation / purification Bacteria - Gram negative Pseudomonas aeruginosa

Get tips on using MICROBExpress™ Bacterial mRNA Enrichment Kit to perform RNA isolation / purification Bacteria - Gram negative Pseudomonas aeruginosa

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.