Microarray Gene expression arrays Rhesus monkey brain tissue

Get tips on using Flotillin-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - A2780 FLOT1

Get tips on using connexin 43 siRNA (r) to perform siRNA / miRNA gene silencing Rat - Pericytes Cx43

Get tips on using Dlx-2 siRNA (h) to perform siRNA / miRNA gene silencing Human - A549 DLX2

Get tips on using siGENOME Human PAK1 siRNA to perform siRNA / miRNA gene silencing Human - HeLa PAK1

Get tips on using ON-TARGETplus SMARTpool - Human to perform siRNA / miRNA gene silencing Human - U2OS DKC1

Get tips on using SnoA/N siRNA (h) to perform siRNA / miRNA gene silencing Human - SW1990 SnoN

Get tips on using Senescence Detection Kit - Biovision to perform Reporter gene assay β-galactosidase substrates - H9C2

Get tips on using X-Gal Staining Kit to perform Reporter gene assay β-galactosidase substrates - C2C12

Get tips on using Senescence Detection Kit - Biovision to perform Reporter gene assay β-galactosidase substrates - Huh7

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.