DNA transfection Mammalian cells Primary cells

Get tips on using pANC232 (xylF) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylF

Get tips on using pANC231 (xylE) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylE

Get tips on using pANC230 (xylD) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylD

Get tips on using pANC223 (xylC) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylC

Get tips on using pANC210 (xylB) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylB

Get tips on using pANC209 (xylA) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylA

Get tips on using STEMdiff™ Trilineage Differentiation Kit to perform Stem cell Differentiation media Differentiation of Human primed induced pluripotent stem cells (UMN PCBC16iPS) into naive pluripotent stem cells

Generally it has been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Use of enzymes to disrupt cell wall can alter gene expression profiles. Therefore, physical disruption can result in high quality RNA for all downstream processing. Use of DNAse and proteinase K will remove traces of DNA contamination and proteins respectively.

Generally it has been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Use of enzymes to disrupt cell wall can alter gene expression profiles. Therefore, physical disruption can result in high quality RNA for all downstream processing. Use of DNAse and proteinase K will remove traces of DNA contamination and proteins respectively.

Generally it has been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Use of enzymes to disrupt cell wall can alter gene expression profiles. Therefore, physical disruption can result in high quality RNA for all downstream processing. Use of DNAse and proteinase K will remove traces of DNA contamination and proteins respectively.