siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1

Get tips on using E-Gel™ 1 Kb Plus Express DNA Ladder to perform DNA Ladder 1 kb

Get tips on using Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit to perform ELISA Mouse - IL-1 beta

Get tips on using Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit to perform ELISA Rat - IL-1 beta

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using Anti-UCP-1 antibody produced in rabbit to perform Immunohistochemistry Mouse - UCP1

Get tips on using PARP-1 Antibody (F-2): sc-8007 to perform Western blotting PARP

Get tips on using Notch 1 Antibody (E-4): sc-373944 to perform Western blotting Notch1

Get tips on using Rat MCP-1 PicoKine™ ELISA Kit to perform ELISA Rat - MCP1

Get tips on using Rat JE/MCP-1/CCL2 DuoSet ELISA to perform ELISA Rat - MCP1

Get tips on using β-Galactosidase Reporter Gene Staining Kit to perform Reporter gene assay β-galactosidase substrates - rat mesenchymal stem cells (MSCs)