siRNA / miRNA gene silencing Rat Brain endothelial cells

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Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Cells Immortalized cell lines SH-SY5Y

Cell culture media 3D Cell Culture Media PLC/PRF/5 cells-spheres

Get tips on using MagMAX™-96 Total RNA Isolation Kit to perform RNA isolation / purification Tissue - rat heart tissue

Products Thermo Fisher Scientific MagMAX™-96 Total RNA Isolation Kit

Get tips on using MagMAX™-96 Total RNA Isolation Kit to perform RNA isolation / purification Tissue - rat lung tissue

Products Thermo Fisher Scientific MagMAX™-96 Total RNA Isolation Kit

Get tips on using MagMAX™-96 Total RNA Isolation Kit to perform RNA isolation / purification Tissue - rat liver tissue

Products Thermo Fisher Scientific MagMAX™-96 Total RNA Isolation Kit

Get tips on using MagMAX™-96 Total RNA Isolation Kit to perform RNA isolation / purification Tissue - rat pancreas tissue

Products Thermo Fisher Scientific MagMAX™-96 Total RNA Isolation Kit

Get tips on using TdT In Situ Apoptosis Detection Kit - Fluorescein to perform TUNEL assay cell type - Rat fibroblast-like synoviocytes

Products R&D system, Minneapolis, MN, USA TdT In Situ Apoptosis Detection Kit - Fluorescein

Get tips on using Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade to perform ChIP H3K36Me3 - Sheep Rat -NA-

Products Abcam Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade

Get tips on using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005 to perform ChIP Rat - Mesenteric arteries

Products Cell Signaling Technology SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - H9c2 rat cardiomyocytes

Products Enzo Life Sciences ROS-ID® Total ROS/Superoxide detection kit

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