siRNA / miRNA gene silencing Rat IEC-6

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using CM-H2DCFDA (General Oxidative Stress Indicator) to perform ROS assay cell type - PC12

Get tips on using CM-H2DCFDA (General Oxidative Stress Indicator) to perform ROS assay cell type - T47D

Get tips on using GeneJuice® Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines 3T3-L1

Get tips on using Anti-Calretinin Antibody, clone 6B8.2 to perform Immunohistochemistry Mouse - Calb2

Get tips on using Fibronectin Antibody (617): sc-81769 to perform Western blotting Fibronectin

Get tips on using Vimentin Antibody (5G3F10): sc-66002 to perform Western blotting Vimentin

Get tips on using GAPDH Antibody (6C5): sc-32233 to perform Western blotting GAPDH

Get tips on using Agilent RNA 6000 Pico Kit to perform RNA quantification Fuorimetric

Get tips on using CM-H2DCFDA (General Oxidative Stress Indicator) to perform ROS assay cell type - CHO-K1