Site Directed Mutagenesis (SDM) Human Point mutation THP-1

Get tips on using VEGF-D siRNA (h) to perform siRNA / miRNA gene silencing Human - Caki-2 VEGF-D

Get tips on using IL-8 siRNA (h) to perform siRNA / miRNA gene silencing Human - HUVEC IL-8 Lipid

Get tips on using FlexiTube GeneSolution GS7052 for TGM2 to perform siRNA / miRNA gene silencing Human - Caki-2 TGM2

Get tips on using SignalSilence® SAPK/JNK siRNA to perform siRNA / miRNA gene silencing Human - KGN SAPK/JNK

Get tips on using Direct-zol RNA Kits to perform RNA isolation / purification Cells - primary human renal proximal tubular epithelial cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Thyroid gland

The most widely used method for protein quantification is by spectrophotometry. The concentration of the protein in the samples is measured at an absorbance of 280 nm. The absorbance of the sample protein is then plotted against a standard curve. This method allows for total protein quantification in a sample (cell and tissue extracts). Before analysing the concentration of protein in the sample, it is important to choose the right test method.  For high protein concentration samples (above 5 - 160 mg/ml) the best method is to use the Biuret test. For low concentrations samples (between 1 - 2000µg/ml) the best methods are Lowry assay, BCA assay, Bradford assay and coomassie blue (for exact sensitivity of the test kits you use, refer to manufacturer's protocol). If the samples contain detergents like Triton X-100 then BCA assay is the best choice. For samples that have proteins larger than 3 KDa in size Bradford assay is the best choice. Each method has advantages and disadvantages, plan your analysis considering your sample characteristics.

The most widely used method for protein quantification is by spectrophotometry. The concentration of the protein in the samples is measured at an absorbance of 280 nm. The absorbance of the sample protein is then plotted against a standard curve. This method allows for total protein quantification in a sample (cell and tissue extracts). Before analysing the concentration of protein in the sample, it is important to choose the right test method.  For high protein concentration samples (above 5 - 160 mg/ml) the best method is to use the Biuret test. For low concentrations samples (between 1 - 2000µg/ml) the best methods are Lowry assay, BCA assay, Bradford assay and coomassie blue (for exact sensitivity of the test kits you use, refer to manufacturer's protocol). If the samples contain detergents like Triton X-100 then BCA assay is the best choice. For samples that have proteins larger than 3 KDa in size Bradford assay is the best choice. Each method has advantages and disadvantages, plan your analysis considering your sample characteristics.

Get tips on using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 to perform ChIP Human - SH-SY5Y

Get tips on using Fenozol to perform RNA isolation / purification Tissue - human aorta tissue