Get tips on using ProtoScript® II First Strand cDNA Synthesis Kit to perform cDNA synthesis Tissue
Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - Colorectal aenocarenoma (SW48) - paraffin embeded
Get tips on using Live-Dead cell staining kit (Enzo) to perform Live / Dead assay mammalian cells - human fibroblast tissue
The most widely used method for protein quantification is by spectrophotometry. The concentration of the protein in the samples is measured at an absorbance of 280 nm. The absorbance of the sample protein is then plotted against a standard curve. This method allows for total protein quantification in a sample (cell and tissue extracts). Before analysing the concentration of protein in the sample, it is important to choose the right test method. For high protein concentration samples (above 5 - 160 mg/ml) the best method is to use the Biuret test. For low concentrations samples (between 1 - 2000µg/ml) the best methods are Lowry assay, BCA assay, Bradford assay and coomassie blue (for exact sensitivity of the test kits you use, refer to manufacturer's protocol). If the samples contain detergents like Triton X-100 then BCA assay is the best choice. For samples that have proteins larger than 3 KDa in size Bradford assay is the best choice. Each method has advantages and disadvantages, plan your analysis considering your sample characteristics.
Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - Mouse liver tissue
Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Rat - Trigeminal ganglia tissue
Get tips on using Ribo-Zero® rRNA Removal Kit to perform RNA quantification Fuorimetric - human brain tissue
Get tips on using Pierce™ Coomassie (Bradford) Protein Assay Kit to perform Protein quantification Tissue - mouse liver
Get tips on using Pierce™ Coomassie (Bradford) Protein Assay Kit to perform Protein quantification Tissue - mouse kidney
Get tips on using Pierce™ Coomassie (Bradford) Protein Assay Kit to perform Protein quantification Tissue - mouse brain
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