siRNA / RNAi /miRNA transfection Human Cells Cal 27 cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat brain microvascular endothelial cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat aortic smooth muscle cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary porcine coronary artery endothelial cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary bovine pulmonary artery endothelial cells

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary porcine airway epithelial cells

Get tips on using QIAGEN Proteinase K (10 ml) to perform RNA isolation / purification Cells - primary mouse morula cells

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.