siRNA / miRNA gene silencing Human UCC (urothelial cancer cell lines)

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - A549 human adenocarcinomic human alveolar basal epithelial cells

Get tips on using PI/RNASE Solution to perform Cell cycle assay human - U266

Get tips on using PI/RNASE Solution to perform Cell cycle assay human - Jurkat

Get tips on using PI/RNASE Solution to perform Cell cycle assay human - K562

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Cell Proliferation ELISA, BrdU to perform Cell cytotoxicity / Proliferation assay cell type - FADU

Get tips on using Cell Counting Kit-8 to perform Cell cytotoxicity / Proliferation assay cell type - HeLa

Get tips on using Cell Proliferation ELISA, BrdU to perform Cell cytotoxicity / Proliferation assay cell type - HeLa