Get tips on using Cellstain-Double Staining Kit to perform Live / Dead assay mammalian cells - HepaRG human hepatoma
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Get tips on using connexin 43 ShRNA to perform shRNA gene silencing Human - Neuroblastoma cells (SH-SY5Y) Connexin 43 lentiviral particles
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Human Spinal cord
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Human Pituitary gland
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Human Fallopian tubes
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - human liver tissue
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - human kidney tissue
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - human brain tissue
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