siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using MethylCap kit to perform DNA methylation profiling Whole genome profiling - HCT116, HTC15 human colon cancer cells

Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform Stem cell culture media Human Fetal brain-derived neural stem cells

Get tips on using Xfect™ Transfection Reagent to perform siRNA / RNAi /miRNA transfection Rat - H9c2 Cationic and neutral lipids

Get tips on using Live-Dead Cell Staining Kit (BioVision) to perform Live / Dead assay mammalian cells - MDA-MB-231 human breast cancer cells

Get tips on using LIVE/DEAD™ Cell Imaging Kit to perform Live / Dead assay mammalian cells - MDA-MB-231 human breast cancer cells

Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media Human intestinal stem cells/organoids

Get tips on using Cytoselect™ Cell Viability and Cytotoxicity Assay to perform Live / Dead assay mammalian cells - MCF-7 human breast cancer cells

Get tips on using HyClone Minimal Essential Medium (MEM) variations: Liquid to perform Stem cell culture media Human salivary gland stem cells

Get tips on using Gibco DMEM/F-12, HEPES to perform 3D Cell Culture Media Mouse primary mammary ephitelial cells- organoids