Protein expression and purification Insect cells S2

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human peripheral blood monocytes

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human fibroblast-like synoviocytes

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human chondrocytes - rheumatoid arthritis

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - primary mouse cortical neurons

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Cells - primary mouse cardiac fibroblasts

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat hippocampal neurons

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat epidermal keratinocytes

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat dermal fibroblasts