siRNA / miRNA gene silencing Rat Brain endothelial cells

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using GeneJET RNA Purification Kit to perform RNA isolation / purification Cells - primary human renal artery smooth muscle cells

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - Rat_Mesenteric fat

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - Human ovarian tissue MGMT

Get tips on using Beta-Galactosidase Staining Kit to perform Reporter gene assay β-galactosidase substrates - INS-1 832/13

Get tips on using β-Galactosidase Staining Kit to perform Reporter gene assay β-galactosidase substrates - human knee bone tissue

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - Whole blood (human) FKBP5

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - Hypothalamus mouse tissue MeCP2

Get tips on using EZ DNA Methylation-Gold Kit to perform DNA methylation profiling Gene specific profiling - MRC-5 Actb

Get tips on using CpGenome Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - MG-63 PTEN