siRNA / miRNA gene silencing Rat Brain endothelial cells HIF-1α

Get tips on using EZ DNA Methylation-Gold Kit to perform DNA methylation profiling Gene specific profiling - MCF-7 BMP2

Get tips on using Beta-Lactamase Activity Assay Kit to perform Reporter gene assay β-lactamase substrates - Burkholderia cepacia complex

Get tips on using MethylMiner™ Methylated DNA Enrichment Kit to perform DNA methylation profiling Gene specific profiling - SKOV3 ZIC1

Get tips on using EpiTect Fast DNA Bisulfite Kit (50) to perform DNA methylation profiling Gene specific profiling - Hep3B SPRY1

Get tips on using Negative control, native pGL4.13 luciferase vector to perform Reporter gene assay luciferase - negative control (luciferase vector)

Get tips on using Secrete-Pair™ Dual Luminescence Assay Kit to perform Reporter gene assay β-galactosidase substrates - HUVEC

Get tips on using GenElute™ Mammalian Total RNA Miniprep Kit to perform RNA isolation / purification Cells - primary human pancreatic stellate cells

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using Anti-p62/SQSTM1 antibody produced in rabbit to perform Autophagy assay cell type - Hippocampal neural stem cells

Get tips on using Senescence Detection Kit - Merck to perform Reporter gene assay β-galactosidase substrates - MCF-7 human breast cancer