protein-isolation-tissue-mouse-heart

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using pET-32c to perform Protein Expression Prokaryotic cells - E. coli rpf-like protein

Get tips on using EMBacY#5 to perform Protein Expression Eukaryotic cells - Hi5 Soluble G protein (Hendra Virus)

Get tips on using pTip-QC2-gi_21218674 to perform Protein Expression Prokaryotic cells - R. erythropolis putative DNA-binding protein

Get tips on using Tetro cDNA Synthesis Kit to perform cDNA synthesis Tissue

Get tips on using qScript cDNA Synthesis Kit to perform cDNA synthesis Tissue

Get tips on using QuantiTect Reverse Transcription Kit to perform cDNA synthesis Tissue

Get tips on using iScript cDNA Synthesis Kit to perform cDNA synthesis Tissue

Get tips on using pET30a(+)-karp to perform Protein Expression Prokaryotic cells - E. coli 56‐kDa O. tsutsugamushi strain Karp protein