siRNA / RNAi /miRNA transfection Human Cal 27 cells

Get tips on using Senescence Cells Histochemical Staining Kit to perform Reporter gene assay β-galactosidase substrates - A549

Get tips on using DeadEnd™ Fluorometric TUNEL System to perform TUNEL assay cell type - HeLa cells human cervical cancer

Get tips on using Anti-LC3 antibody produced in rabbit to perform Autophagy assay cell type - Human osteosarcoma cancer cells

Get tips on using ARCTURUS® PicoPure® DNA Extraction Kit to perform RNA isolation / purification Cells - primary human melanocytes

Get tips on using Senescence Cells Histochemical Staining Kit to perform Reporter gene assay β-galactosidase substrates - Aspc-1

Get tips on using Senescence Cells Histochemical Staining Kit to perform Reporter gene assay β-galactosidase substrates - Bxpc-3

Get tips on using PrimaPure Porcine Endothelial Cells Growth Medium to perform Mammalian cell culture media PAOEC

Get tips on using miRNeasy Serum/Plasma Kit to perform RNA isolation / purification Tissue - Human Blood / Serum / Plasma / Buffy coat

Get tips on using Senescence Cells Histochemical Staining Kit to perform Reporter gene assay β-galactosidase substrates - mouse embryonic fibroblasts

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.