Protein Expression Eukaryotic cells N. benthamiana

Get tips on using N-WASP siRNA (h) to perform siRNA / miRNA gene silencing Human - T47-D N-WASP

Get tips on using HeV NCORE pET43.1a to perform Protein Expression Prokaryotic cells - E. coli HeV N

Get tips on using pTSara-NatB to perform Protein Expression Prokaryotic cells - E. coli N-terminal acetyltransferase B

Get tips on using N-cadherin Antibody (8C11): sc-53488 to perform Western blotting N‐cadherin

Get tips on using Anti-N-Cadherin Antibody, clone 13A9 to perform Western blotting N‐cadherin

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Get tips on using ATG5 Antibody (N-term) to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using SnoA/N siRNA (h) to perform siRNA / miRNA gene silencing Human - SW1990 SnoN

Get tips on using Bax antibody [N1N2], N-term to perform Western blotting Bax

Get tips on using Mouse Shh-N ELISA Kit to perform ELISA Mouse - ShhN